what will happend?

i just upgrade 1.4.1 akaio to 1.5 and i put my mario and luigi bowser inside story (patch rom), could this affect in something? cause i played it and works great

I don't think so. Newer flashcart firmware should have a higher capability to play roms than its older version. But you should backup the v1.4.1 in case something happens. :)

but then tell me what could happend?should i delet this rom and dump a clean one? or keep the one i have
?

Don't delete anything, you don't need too. Just replace the 1.4.1 firmware with the 1.5 one. & like kamui said, backup the 1.4.1 firmware if your not sure.

look, i already updated with 1.5 but my roms like gta and mario luigi bowser inside story where patch roms, but they work fine!!! should i do something? if i leave it like that will affect my flashcart?

updating your flashcart firmware will not generally break compatibility with already working roms.

thank you :)